Journal: The Journal of Biological Chemistry

Article Title: Effect of phospholipid transfer protein on plasma sphingosine-1-phosphate

doi: 10.1016/j.jbc.2024.107837

Figure Lengend Snippet: Lipid and PLTP activity measurements in A apo m -KO mice . A, strategy for Apom -KO mouse preparation. Three-month-old mice (male and female) on chow diet were used. B, plasma (0.2 μl) was separated by 4 to 15% SDS gel electrophoresis and immunoblotted with polyclonal antibodies against apoM. Ponceau S staining was used as a loading control . C, plasma cholesterol measurement. D, plasma PLTP activity measurement. E, plasma S1P measurement. The above mice were then put on the Western diet for 2 weeks and the following were measured. F, plasma cholesterol. G, PLTP activity. H, plasma S1P. Values are the mean ± SD, n = 4 to 5, ∗ p < 0.01. PLTP, plasma phospholipid transfer protein; S1P, sphingosine-1-phosphate.

Article Snippet: Plasma (0.2 μl) was separated by 4 to 15% SDS gel electrophoresis and immunoblotted with polyclonal antibodies against apoM (Proteintech, Catalog #:12817-1-AP) and apoA-I (Proteintech, Catalog #:14427-1-AP).

Techniques: Activity Assay, Clinical Proteomics, SDS-Gel, Electrophoresis, Staining, Control, Western Blot

Journal: The Journal of Biological Chemistry

Article Title: Effect of phospholipid transfer protein on plasma sphingosine-1-phosphate

doi: 10.1016/j.jbc.2024.107837

Figure Lengend Snippet: S1P and apoM measurement in Alb-KO mice . Three-month-old Alb -KO- male mice on chow diet were used. A, Western blot analyses of plasma albumin and apoM. B, plasma S1P measurement. C, PLTP activity measurement. Values are the mean ± SD, n = 3. Alb -KO, albumin gene knockout mice; PLTP, plasma phospholipid transfer protein; S1P, sphingosine-1-phosphate.

Article Snippet: Plasma (0.2 μl) was separated by 4 to 15% SDS gel electrophoresis and immunoblotted with polyclonal antibodies against apoM (Proteintech, Catalog #:12817-1-AP) and apoA-I (Proteintech, Catalog #:14427-1-AP).

Techniques: Western Blot, Clinical Proteomics, Activity Assay, Gene Knockout

Journal: The Journal of Biological Chemistry

Article Title: Effect of phospholipid transfer protein on plasma sphingosine-1-phosphate

doi: 10.1016/j.jbc.2024.107837

Figure Lengend Snippet: Lipid and apolipoprotein measurement in inducible Pltp - KO mice . Three-month-old mice (male and female) on chow diet were used. A, plasma PLTP activity measurement. B, FPLC for evaluating plasma cholesterol distribution. HDL and non-HDL fractions were labeled. C, Western blot analyses for plasma apoA-I and apoM (male). The intensity of each band, measured by Image-Pro Plus version 4.5 software, was used for quantification. For apoA-I: 100 ± 7% WT versus 55 ± 6% Pltp -KO, p < 0.01; for apoM: 100 ± 15% WT versus 44 ± 5% Pltp -KO, p < 0.01. D, plasma cholesterol measurement. E, plasma phospholipid measurement. F, plasma S1P measurement (male). Three-month-old mice (male and female) on the Western diet for 2 weeks were used. G, plasma cholesterol measurement. H, plasma phospholipid measurement. I, plasma S1P measurement (male). Values are the mean ± SD, n = 5, ∗∗ p < 0.01. FPLC, fast protein liquid chromatography; PKO, Pltp -KO mice; PLTP, plasma phospholipid transfer protein; S1P, sphingosine-1-phosphate.

Article Snippet: Plasma (0.2 μl) was separated by 4 to 15% SDS gel electrophoresis and immunoblotted with polyclonal antibodies against apoM (Proteintech, Catalog #:12817-1-AP) and apoA-I (Proteintech, Catalog #:14427-1-AP).

Techniques: Clinical Proteomics, Activity Assay, Labeling, Western Blot, Software, Fast Protein Liquid Chromatography

Journal: The Journal of Biological Chemistry

Article Title: Effect of phospholipid transfer protein on plasma sphingosine-1-phosphate

doi: 10.1016/j.jbc.2024.107837

Figure Lengend Snippet: Plasma lipid measurement in experimental and WT mice . WT, Apom -KO (MKO), inducible Pltp -KO (PKO), and inducible Pltp -KO/germline Apom -KO (dKO) mice on chow diet were used. A, plasma cholesterol measurement in male mice. B, plasma phospholipid measurement in male mice. C, plasma S1P measurement in male mice. D, plasma S1P measurement in female mice. Values are the mean ± SD, n = 4. Columns labeled with different lowercase letters are statistically different, p < 0.01. dKO, double KO; PLTP, plasma phospholipid transfer protein; S1P, sphingosine-1-phosphate.

Article Snippet: Plasma (0.2 μl) was separated by 4 to 15% SDS gel electrophoresis and immunoblotted with polyclonal antibodies against apoM (Proteintech, Catalog #:12817-1-AP) and apoA-I (Proteintech, Catalog #:14427-1-AP).

Techniques: Clinical Proteomics, Labeling

Journal: The Journal of Biological Chemistry

Article Title: Effect of phospholipid transfer protein on plasma sphingosine-1-phosphate

doi: 10.1016/j.jbc.2024.107837

Figure Lengend Snippet: PLTP activity, lipid, and apoM measurements in germline Pltp - transgenic mice . Three-month-old Pltp -Tg and WT male mice on chow diet were used. A , PLTP activity measurement. B, plasma cholesterol measurement. C, plasma phospholipid measurement. D, plasma S1P measurement. E, Western blot analyses for plasma apoM. The intensity of each band was used for quantification: 100 ± 5% WT versus 95 ± 15% Pltp -Tg. Values are the mean ± SD, n = 4. ∗ p < 0.05, ∗∗ p < 0.01. PLTP, plasma phospholipid transfer protein; S1P, sphingosine-1-phosphate.

Article Snippet: Plasma (0.2 μl) was separated by 4 to 15% SDS gel electrophoresis and immunoblotted with polyclonal antibodies against apoM (Proteintech, Catalog #:12817-1-AP) and apoA-I (Proteintech, Catalog #:14427-1-AP).

Techniques: Activity Assay, Transgenic Assay, Clinical Proteomics, Western Blot

Journal: The Journal of Biological Chemistry

Article Title: Effect of phospholipid transfer protein on plasma sphingosine-1-phosphate

doi: 10.1016/j.jbc.2024.107837

Figure Lengend Snippet: Lipid and apolipoprotein measurements in AAV- Pltp and AAV-null mice . Two-month-old WT male mice (C57BL/6) were injected with AAV-PLTP and AAV-null (i.v., 2 × 10 11 per 100 ul). Three weeks later, lipid and apolipoprotein levels were measured. A, PLTP activity measurement. B, plasma cholesterol measurement. C, plasma phospholipid measurement. D, Western blot analyses for plasma apoA-I and apoM. The intensity of each band was used for quantification. For apoA-I: 100 ± 10% WT versus 65 ± 15% AAV-PLTP, p < 0.05; for apoM: 100 ± 8% WT versus 52 ± 15% AAV-PLTP, p < 0.01. E, plasma S1P measurement. Values are the mean ± SD, n = 4 to 5. ∗∗ p < 0.01. AAV, adenovirus-associated virus; PLTP, plasma phospholipid transfer protein; S1P, sphingosine-1-phosphate.

Article Snippet: Plasma (0.2 μl) was separated by 4 to 15% SDS gel electrophoresis and immunoblotted with polyclonal antibodies against apoM (Proteintech, Catalog #:12817-1-AP) and apoA-I (Proteintech, Catalog #:14427-1-AP).

Techniques: Injection, Activity Assay, Clinical Proteomics, Western Blot, Virus

Journal: Antibiotics

Article Title: Protective Effect of Willow ( Salix babylonica L.) on Fish Resistance to Vibrio parahaemolyticus and Vibrio alginolyticus

doi: 10.3390/antibiotics12060989

Figure Lengend Snippet: Minimal inhibition concentration (MIC) and minimal bactericidal concentration (MBC) of LL2 and LL3 against V. parahaemolyticus and V. alginolyticus .

Article Snippet: Specifically, after 24 h of culture, the bacterial densities of V. alginolyticus ATCC 17749 were 10 8.93 , 10 6.4 , 10 5.04 , and 10 2.03 CFU/mL, and the densities of the V. alginolyticus NG20 strain were 10 8.95 , 10 6.04 , 10 5.15 , and 10 1.96 CFU/mL corresponding to the treatment doses of 0.25 × MIC, 0.5 × MIC, 1.0 × MIC, and 2.0 × MIC ( and ); the survival rates of V. alginolyticus ATCC 17749 were 85.77%, 61.43%, 48.41%, and 19.52%, and those of V. alginolyticus NG20 were 82.36%, 55.61%, 47.37%, and 18.08%, respectively, for the above treatment doses ( ).

Techniques: Inhibition, Concentration Assay, Bacteria

Journal: Antibiotics

Article Title: Protective Effect of Willow ( Salix babylonica L.) on Fish Resistance to Vibrio parahaemolyticus and Vibrio alginolyticus

doi: 10.3390/antibiotics12060989

Figure Lengend Snippet: Time-kill kinetics of LL3 against V. alginolyticus ATCC 17749.

Article Snippet: Specifically, after 24 h of culture, the bacterial densities of V. alginolyticus ATCC 17749 were 10 8.93 , 10 6.4 , 10 5.04 , and 10 2.03 CFU/mL, and the densities of the V. alginolyticus NG20 strain were 10 8.95 , 10 6.04 , 10 5.15 , and 10 1.96 CFU/mL corresponding to the treatment doses of 0.25 × MIC, 0.5 × MIC, 1.0 × MIC, and 2.0 × MIC ( and ); the survival rates of V. alginolyticus ATCC 17749 were 85.77%, 61.43%, 48.41%, and 19.52%, and those of V. alginolyticus NG20 were 82.36%, 55.61%, 47.37%, and 18.08%, respectively, for the above treatment doses ( ).

Techniques:

Journal: Antibiotics

Article Title: Protective Effect of Willow ( Salix babylonica L.) on Fish Resistance to Vibrio parahaemolyticus and Vibrio alginolyticus

doi: 10.3390/antibiotics12060989

Figure Lengend Snippet: Time-kill kinetics of LL3 against V. alginolyticus NG20.

Article Snippet: Specifically, after 24 h of culture, the bacterial densities of V. alginolyticus ATCC 17749 were 10 8.93 , 10 6.4 , 10 5.04 , and 10 2.03 CFU/mL, and the densities of the V. alginolyticus NG20 strain were 10 8.95 , 10 6.04 , 10 5.15 , and 10 1.96 CFU/mL corresponding to the treatment doses of 0.25 × MIC, 0.5 × MIC, 1.0 × MIC, and 2.0 × MIC ( and ); the survival rates of V. alginolyticus ATCC 17749 were 85.77%, 61.43%, 48.41%, and 19.52%, and those of V. alginolyticus NG20 were 82.36%, 55.61%, 47.37%, and 18.08%, respectively, for the above treatment doses ( ).

Techniques:

Journal: Antibiotics

Article Title: Protective Effect of Willow ( Salix babylonica L.) on Fish Resistance to Vibrio parahaemolyticus and Vibrio alginolyticus

doi: 10.3390/antibiotics12060989

Figure Lengend Snippet: Survival rate of V. alginolyticus ATCC 17749 và V. alginolyticus NG20 following treatment with LL3.

Article Snippet: Specifically, after 24 h of culture, the bacterial densities of V. alginolyticus ATCC 17749 were 10 8.93 , 10 6.4 , 10 5.04 , and 10 2.03 CFU/mL, and the densities of the V. alginolyticus NG20 strain were 10 8.95 , 10 6.04 , 10 5.15 , and 10 1.96 CFU/mL corresponding to the treatment doses of 0.25 × MIC, 0.5 × MIC, 1.0 × MIC, and 2.0 × MIC ( and ); the survival rates of V. alginolyticus ATCC 17749 were 85.77%, 61.43%, 48.41%, and 19.52%, and those of V. alginolyticus NG20 were 82.36%, 55.61%, 47.37%, and 18.08%, respectively, for the above treatment doses ( ).

Techniques:

Journal: Antibiotics

Article Title: Protective Effect of Willow ( Salix babylonica L.) on Fish Resistance to Vibrio parahaemolyticus and Vibrio alginolyticus

doi: 10.3390/antibiotics12060989

Figure Lengend Snippet: Survival rate of LL3-treated zebrafish challenged with V. alginolyticus NG20.

Article Snippet: Specifically, after 24 h of culture, the bacterial densities of V. alginolyticus ATCC 17749 were 10 8.93 , 10 6.4 , 10 5.04 , and 10 2.03 CFU/mL, and the densities of the V. alginolyticus NG20 strain were 10 8.95 , 10 6.04 , 10 5.15 , and 10 1.96 CFU/mL corresponding to the treatment doses of 0.25 × MIC, 0.5 × MIC, 1.0 × MIC, and 2.0 × MIC ( and ); the survival rates of V. alginolyticus ATCC 17749 were 85.77%, 61.43%, 48.41%, and 19.52%, and those of V. alginolyticus NG20 were 82.36%, 55.61%, 47.37%, and 18.08%, respectively, for the above treatment doses ( ).

Techniques:

Journal: Antibiotics

Article Title: Protective Effect of Willow ( Salix babylonica L.) on Fish Resistance to Vibrio parahaemolyticus and Vibrio alginolyticus

doi: 10.3390/antibiotics12060989

Figure Lengend Snippet: Survival rate of LL3-treated sea bass challenged with V. alginolyticus NG20.

Article Snippet: Specifically, after 24 h of culture, the bacterial densities of V. alginolyticus ATCC 17749 were 10 8.93 , 10 6.4 , 10 5.04 , and 10 2.03 CFU/mL, and the densities of the V. alginolyticus NG20 strain were 10 8.95 , 10 6.04 , 10 5.15 , and 10 1.96 CFU/mL corresponding to the treatment doses of 0.25 × MIC, 0.5 × MIC, 1.0 × MIC, and 2.0 × MIC ( and ); the survival rates of V. alginolyticus ATCC 17749 were 85.77%, 61.43%, 48.41%, and 19.52%, and those of V. alginolyticus NG20 were 82.36%, 55.61%, 47.37%, and 18.08%, respectively, for the above treatment doses ( ).

Techniques: